only a single chromosome (chr22) and the ERCC spike-in, that is the human GRCh38 version of the genome from Ensembl.
chr22_with_ERCC92.gtf
annotations obtained from Ensembl (Homo_sapiens.GRCh38.86.gtf.gz) for chromosome 22 only.
gene_GO_anno.txt
GO functional annotation file.
gene_KEGG_anno.txt
KEGG functional annotation file.
*.fastq
The test data consists of two commercially available RNA samples: Universal Human Reference (UHR)and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR is total RNA isolated from the brains of 23 Caucasians, male and female, of varying age but mostly 60-80 years old.
In addition, a spike-in control was used. Specifically we added an aliquot of the ERCC ExFold RNA Spike-In Control Mixes to each sample. The spike-in consists of 92 transcripts that are present in known concentrations across a wide abundance range (from very few copies to many copies).
